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Sample Preparation

2IP

2IP helps you to prepare your samples for imaging

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-Ìýby entrusting them to our two technicians

- or by using the Histo-lab infrastructure yourself (except for primary antibodies and samples, all equipment and reagents are available)Ìý

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Proposed services and available equipment:

  • Paraffin sections

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Microtomes Microm HM 340 E; HM 355 S ER; Microm HM 340 E equiped withÌýSection TransferÌýSystem (STS) and Cool-Cut

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  • Cryosections

cryostat Leica CM1950

  • Histological staining

HE, trichrome de Masson, picro-Sirius red, PAS, Oil red O, rouge Soudan)

  • Chromogenic Immunohistochemistry (IHC-DAB), multiplex immunofluorescence (TSA method)

Leica Bond RXm autostainer ()

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InformationÌýand advices:Ìý

AdviceÌýfor the design of your immunostainings

How to choose a primary antibody?Ìý

Standard immunostaining protocols

Standard histological staining protocols

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Interested by these services?ÌýFeel free toÌýcontact us

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